Version-1 (Nov–Dec 2016)
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| Paper Type | : | Research Paper |
| Title | : | Production and Optimization of Protease from Aspergillus niger and Bacillus subtilis using Response Surface Methodology |
| Country | : | Nigeria |
| Authors | : | M. A. Milala || I. A. Jatau || A. A. Abdulrahman |
Abstract: Proteases are enzymes that perform proteolysis. In this study, Response Surface Methodology was used for the production of protease from Aspergillus niger and Bacillus subtilis. This was achieved by isolating the Fungus and Bacterium from contaminated soil and bovine intestinal sample and screening for protease activity was carried out using casein as substrate. Optimization of different conditions for maximum protease activity (pH, Temperature, Carbon source and Mineral Salts) and Response of microorganisms on mineral salts in the culture medium was observed. The time course for the production of protease by both isolates was found to be maximum at 48 hours.............
Key words: Protease; Casein;Aspergillus niger; Bacillus subtilis; Response surface methodology
[1]. Abbas, A.S and Leila J (2011) Effect of the culture conditions on the productionof an extracellular protease by Bacillus sp isolated from soil sample ofLavizanJungle Park. Journal of Applied Microbiology.
[2]. Godfrey and West (1996)an overview on fermentation, downstream processingandproperties of microbial proteases. Applied Microbiology and Biotechnology: 60, 381-395.
[3]. Gupta. R, Beg Q.K, Khan S and Chauhan B. (2002). An overview on fermentation, downstream processing and properties of microbialproteases. Appl Microbial Biotechnol: 60, 381-395.
[4]. Gupta, R, Q.K Beg and P. Lorenz (2002). Applied Microbiology andBiotechnology 59,1, 15-32.
[5]. Nunes, A.S, and M. L. L. Martins (2001) "Isolation, properties and kinetics ofgrowth of a thermophilic Bacillus," Brazilian Journalof Microbiology, vol. 32, no. 4, pp. 271–275.
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| Paper Type | : | Research Paper |
| Title | : | Effect of Some Antioxidants on Some Biochemical Parameters and Growth Performance of Nile Tilapia (Oreochromis Niloticus) |
| Country | : | Nigeria |
| Authors | : | Abdel-Aziz, F. ABDEL-AZIZ || Neven, S. || Amany A. El-Shebly |
Abstract: The present study was conducted to evaluate the effect of antioxidants (vitamin C, vitamin E and Selenium) added to the basal diet on some blood parameters and growth performance of Nile tilapia (Oreochromis niloticus). Basal diet (25% protein) was formulated as control. The experiment design included 8 treatments in duplicates Antioxidants were added to the basal diet as follow (1- control (basal diet), 2-basal diet+ vit C, 3-basal diet+ Vit E, 4-basal diet + Se, 5-basal diet + vitamins C& E, 6-basal diet +vitamins C &Se, 7-basal diet +vitamins E& Se and 8-basal diet + vitamins C, E &Se)...............
Key words: Nile tilapia, antioxidants, growth performance, blood parameters
[1]. (APHA) American Public Health Association, American Water Works Association, Water Pollution Control Federation, & Water Environment Federation. (1915). Standard methods for the examination of water and wastewater (Vol. 2). American Public Health Association.
[2]. Acharya, G. and Mohonty, P.,(2012): hematological and serum biochemical parameters in different sexes of walking cat fish, Int. J. Sci. Res. (IJSR) 1914-1917.
[3]. Adham, K. G., Hashem, H. O., Abu-Shabana, M. B., & Kamel, A. H. (2000). Vitamin C deficiency in the catfish Clarias gariepinus. Aquaculture Nutrition,6(2), 129.
[4]. Ai, Q., Mai, K., Tan, B., Xu, W., Zhang, W., Ma, H., & Liufu, Z. (2006). Effects of dietary vitamin C on survival, growth, and immunity of large yellow croaker, Pseudosciaena crocea. Aquaculture, 261(1), 327-336.
[5]. AOAC (Association of official analytical chemists) requirements of juvenile milkfish for essential amino acids. J. Nut. 123:125-132.
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| Paper Type | : | Research Paper |
| Title | : | Evaluation and Selection of Autochthonous Lactic Acid Bacteria as Starter Cultures from Goat's Raw Milk |
| Country | : | Morocco |
| Authors | : | Zaaraoui L. || Ounine K. || Elhamdani M. || Benali A || Oukassou M. || Bouksaim M |
Abstract: A total of twenty five indigenous lactic acid bacteria (LAB) isolates were collected from raw goat's milk Oulmes breed in AIT Ichou region (located in the Middle Atlas, Morocco). Among these twenty-five isolates, seven strains of LAB were selected for their significant acidifying activity and were identified using 16S rRNA gene sequencing. The genetic analysis revealed that all the strains were identified as Lactococcus lactis. These strains have shown some interesting technological properties, as a notable proteolytic activity especially C24 (2.37±0.03mMGlycine)...............
Key words: Lactic acid bacteria; goat's milk; lactococcus lactis; acidifying activity; starter culture
[1]. Benlekhal A., 2005. Situation actuelle du secteur caprin au Maroc et stratégie de développement. Foire caprine du Sud. Essaouira, Maroc.
[2]. Anonyme : FAO STAT, Online data base, 2000.
[3]. Chatibi (S.) - le 9 mai 2011 la filière viande bovine au Maroc quelle place pour l‟élevage traditionnel et quelles bases de qualification pour la viande locale
[4]. Benayad N., (2008). Les huiles essentielles extraites des plantes médicinales marocaines : moyen efficace de lutte contre les ravageurs des denrées alimentaires stockées. Etude réalisée dans le cadre d‟un programme sur l‟Homme et la Biosphère MAB et UNESCO en collaboration avec l‟Université Mohammed V Agdal, Faculté des Sciences de Rabat (63 pages).
[5]. El Soda, M., Ahmed, N., Omran, N., Osman, G., Morsi, A. (2003) Isolation, identification and selection of lactic acid bacteria cultures for cheese making. The Emirates Journalof Agricultural Sciences,15 (2), pp. 51–71.
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| Paper Type | : | Research Paper |
| Title | : | Possible Ameliorative Effect of Zinc in Inhibiting the Toxicity of Tak as Organochlorine Pesticide on Oreochromis Niloticus |
| Country | : | Egypt. |
| Authors | : | Ghada Taha Taha Belal || Nahed S. Gad || Khalid H. Zaghloul |
Abstract: The influence of sub lethal concentrations of Zinc (4.8 and 9.6 mg/l)and the experimental organochlorine pesticide Tak (1.5 and 3 mg/l)individually and in mixture on Nile tilapia (Oreochromis niloticus)for fifteen days were investigated .The results revealed a significant increase ((P0.05)in liver residual Zinc and Tak than muscle tissue of Oreochromis niloticus , accompanied by a disturbance in fish physiological status with significant increase, in serum glucose level , cortisol hormone ,cholesterol , triglycerides , urea , creatinine , AST and ALT activities. The results also demonstrated synergistic effect of Zinc and Tak on Oreochromis niloticus fish exposed to the different studied sub lethal concentrations for 15 days.
Key words: O.niloticus , Zinc, Tak , serum constituents , Bioaccumulation
[1]. Jaya Sharma, Pollution-a grave threat to the environment, man kind and the living world, Pacific Business Review International 8, 2016,pp164.
[2]. Chandran.R, Sivakumar. A.A, Mohandass.S, and Aruchami. M, Effect of cadmium and zinc on antioxidant enzyme activity in the gastropod, Achatina fulica. Comp Biochem Physiol.140,2005,422:426.
[3]. Dutta. H.M, and Dalal.R, The effect of endosulfan on the ovary of bluegill sunfish: a histopathological study (Lepomis macrochirus sp), International Journal Environ Res. 2, 2008,215-224.
[4]. WHO/FAO/IAEA. World Health Organization. Switzerland: Geneva(1996). Trace Elements in Human Nutrition and Health.
[5]. Shawky. Z. Sabae, and Fatma. A.S. Mohamed, Effect of Environmental Pollution on the Health of Tilapia spp. from Lake Qarun, Global Veterinaria 14 (3),2015, 304-328.
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| Paper Type | : | Research Paper |
| Title | : | Structural Comparisons of the Kinases Involved in Cancer Disease Development |
| Country | : | INDIA |
| Authors | : | Arun S. Kharat || Ashish B. Gulwe |
Abstract: More than 575,000 people die of cancer, and more than 1.5 million people are diagnosed with cancer per year in the US.Cancer is considered to be one of the leading causes of morbidity and mortality worldwide.The financial costs of cancer in the US per year are an estimated $263.8 billion in medical costs and lost productivity.African Americans are more likely to die of cancer than people of any other race or ethnicity.It is believed that cancer risk can be reduced by avoiding tobacco, limiting alcohol intake, limiting UV ray exposure from the sun and tanning beds and maintaining............
Key words: Cancer disease,genes, kinases ,strural comparison swiss pdb viewer
[1]. Judelson HS, Ah-Fong AM. The kinome of Phytophthora infestans reveals oomycete-specific innovations and links to other taxonomic groups. BMC Genomics. 2010 Dec 9;11:700. doi: 10.1186/1471-2164-11-700. PubMed PMID: 21143935; PubMed Central PMCID: PMC3019232.
[2]. Haitina T, Fredriksson R, Foord SM, Schiöth HB, Gloriam DE. The G protein-coupled receptor subset of the dog genome is more similar to that in humans than rodents. BMC Genomics. 2009 Jan 15;10:24. doi: 10.1186/1471-2164-10-24. PubMed PMID: 19146662; PubMed Central PMCID: PMC2651185.
[3]. Anamika K, Bhattacharya A, Srinivasan N. Analysis of the protein kinome of Entamoeba histolytica. Proteins. 2008 May 1;71(2):995-1006. PubMed PMID: 18004777.
[4]. Kazi JU, Kabir NN, Soh JW. Bioinformatic prediction and analysis of eukaryotic protein kinases in the rat genome. Gene. 2008 Feb 29;410(1):147-53. doi:10.1016/j.gene.2007.12.003. Epub 2007 Dec 14. PubMed PMID: 18201844
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| Paper Type | : | Research Paper |
| Title | : | Assessment of Genotoxicity PHB/Norbixin/Ethyleneglycol Membrane by Micronucleus Test and Comet Assay |
| Country | : | Brazil |
| Authors | : | Rayssilane Cardoso de Sousa || Antônio Luiz Martins Maia Filho || Danniel Cabral Leão Ferreira || Fabrício Pires de Moura do Amaral || Samylla Miranda Monte || Kethyma Moreira Fonseca || Luiz Fernando Meneses Carvalho || CharllytonLuis Sena Costa || Deuzuita Santos Oliveira Viana || Vicente GalberFreitas Viana |
Abstract: Biomaterials, synthetic or natural origin substances that replace and / or stimulate biological systems that no longer have their functions, require the biocompatibility characteristics, biodegradability and biofunctionality for the restoration of tissue function, for example. Given the antioxidant potential norbixin (Bixaorellana L.) and biodegradability and biocompatibility of polyhydroxybutyrate (PHB) (natural polymer), aimed to prepare a membrane from these two constituents with the addition of ethylene glycol reagent (PHB/norbixin/ethyleneglycol) to evaluate their genotoxic effects through the micronucleus test and comet assay. For this study.............
Key words: Biomaterials, PHB/norbixin/ethyleneglycol, Micronucleus test, Comet assay.
[1] A. C. Guastaldi, A. H. Aparecida. Fosfatos de cálcio de interesse biológico: importância como biomateriais, propriedades e métodos de obtenção de recobrimentos, Quim Nova, 6, 2010, 1352-1358.
[2] R. S. Yamatogi, S. C. Rahal, J. M. Granjeiro, R. Taga, T. M. Cestari, A. F. M. Lima. Histologia da associação de membranas biológicas de origem bovina implantadas no tecido subcutâneo de ratos.Ciência Rural, 35(4), 2005, 837-842.
[3] A. P. T. Pezzin, C. A. C. Zavaglia, E. A. R. Duek. Estudo da degradação in vitro de Blendas de Poli(p-dioxanona)/Poli(L-Ácido Láctico) (PPD/PLLA) preparadas por evaporação de solvente. Polímeros: Ciência e Tecnologia, 12(4), 2002, 285-294.
[4] M. A. C. Sinhoreti, R. P. Vitti, L. Correr-Sobrinho. Biomateriais na odontologia: panorama atual e perspectivas futuras. Rev Assoc. Paul Cir Dent, 67, 2013, 178-186.
[5] N. Aarthi, K. V. Ramana. Identification and Characterization of Polyhydroxybutyrate producing Bacillus cereus and Bacillus mycoides strains. International Journal of Environmental Sciences 1, 2011, 744-756.
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| Paper Type | : | Research Paper |
| Title | : | Do Mutations Inevitably Affect Microorganism's Metabolites Production in Continuous Cultures |
| Country | : | Belgium. |
| Authors | : | Jacques Thierie |
Abstract: In fifty years, the production processes by microorganisms using continuous cultures have been very moderately imposed in industrial environments. Yet, these practices have significant advantages, both economically and from a pragmatic perspective. The main reason for this lack of interest lies in the fact that the chemostat (as the archetype of continuous culture) is widely used for the study of the biological evolution of microorganisms. Supposed to create a significant evolutionary pressure, these devices have been increasingly considered as generators of mutations able to defectively modify the "good" properties of the continuously grown producing cells. Over time, prejudices were developed to depreciate the value of these production processes. The viewpoint is not entirely unfounded, but we try to take stock of some excessive attitude, sometimes totally indefensible, and to rehabilitate a production process too often irrationally ruled out.
Key words: chemostat, genetic drift, mutations, microorganisms' metabolites production, steady state
[1] Novick A. and Szilard L. Description of the chemostat. Science 112(2921), 1950a,715-716
[2] Monod J. The growth of bacterial cultures. Ann. Rev. Microbiol. 3, 1949, 371-394
[3] Monod J. La technique de culture continue : théorie et applications. Ann. Instit. Pasteur. 79, 1950, 390-410
[4] Zeng A.P. and Sun J. (2010). Continuous Culture. in: Baltz R.H., Demain A.L and Davies J.E (Chief Eds) Bull A.T., Junker B., Katz L., Lynd L.R., Masureckar P., Reeves C.D. and Huimin Zhaa (Eds.) Manual of Industrial Microbiology and Biotechonology.(ASM Press, Washington, DC) 685-699.
[5] Pearson Education Limited and Cogent Unit 2: Industrial Microbiology 2013 http://www. contentextra. com/lifesciences/unit2 /unit2home.aspx.
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| Paper Type | : | Research Paper |
| Title | : | Genome Sequence Analysis to Evaluate the Performance of Pair wise Statistical Significance of Solanum Lycopersicum |
| Country | : | India.. |
| Authors | : | Uma kumari || Ashok Kumar Choudhary |
Abstract:Solanum lycopersicum is a excellent plant for this approach considering the high quality and comparative analysis of genome sequence of tomato .Sequencing the genome of the crop solanum lycopersicum will also help to identify beneficial genes in other plant relative of the tomato such as potato,pepper.All of these crops are members of the solanaceae or nightshade family, one of the worlds most important vegetable plants families in term of both economic value and production volume. Developing better tomatoes will also contribute to the quest for global food security. As well as using this new genome information to develop a wide variety of beneficial traits, the(TGRD)the tomato genomic resources database is an online and interactive relational database developed using open sources software.............
Key words: Bioinformatics, Genome database, TGRD(Tomato genomic resources database),Sequence analysis, Data compiled, Sequence alignment, solanum lycopersicum
[1]. Altschuhen; Gish, Warren; Miller, Webb; Myers, Eugene; Lipman, David (1990). "Basic local alignment search tool". Jol, Stepurnal of Molecular Biology 215 (3): 403–410
[2]. Andreas D.Baxevanis,B.F.Fracis Quellette,‖A practical guide to the analysis of Gene and protein .3RD Edition october 2004.Published by Wiley,John and Sons.
[3]. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: Update. Nucleic Acids Research, 2004, vol 32, Database Issue: D23-D26.
[4]. "ClustalW / ClustalX: Multiple Sequence Alignment". Retrieved 1 October 2013.
[5]. Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD (2003). "Multiple sequence alignment with the Clustal series of programs". Nucleic Acids Res 31 (13): 3497–3500. doi:10.1093/nar/gkg500. PMC 168907. PMID 12824352.
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| Paper Type | : | Research Paper |
| Title | : | High Resolution Melting Curve Analysis: an Efficient Method for Genetic Purity Analysis of Cotton (Gossypium hirsutum) Hybrid and their Parental Lines |
| Country | : | India.. |
| Authors | : | Tejaswini S Ghatage || Pulak Das |
Abstract: Cotton (Gossypium hirsutun) is one of the most important crops in India. It is essential to develop an efficient method for genetic purity analysis of hybrid cotton and their parental lines. Use of morphological differences, between true hybrids and off types in grow out test (GOT) for genetic purity analysis, are not always apparent and cannot be recognised easily. Further, morphological traits are costly, tedious to score and environment sensitive. Alternatively, it is suggested that recent breakthrough in molecular markers can be employed in genetic purity analysis. Gossypium hirsutum Fı hybrid AJ-999 and it's parents P402 as male and T28-II as female were studied for identification and genetic purity testing with high resolution melt analysis and agarose gel electrophoresis. There are 30 cotton microsatellite loci i.e.............
Key words: Agarose gel electrophoresis; Cotton; Genetic purity; Gossypium hirsutun; High resolution melting curve analysis
[1]. P Selvakumar, R Ravikesavan, A Gopikrishnan, K Thiyagu, S Preetha and N Manikanda Boopathi, Genetic purity analysis of cotton (Gossypium spp.) hybrids using SSR markers, Seed Sci. & Technol., 38 (2010) 358-366
[2]. S Mehetre,S Pardeshi, S Pawar, S Gahukar and U Chavan,In ovulo embryo cultured hybrid between Gossypium hirsutum and Gossypium arboreum: hybridity confirmation. Journal of Cotton Research and Development, 21 (2007) 131–139.
[3]. Ashok B Dongre, Mamta P Raut, Manoj R Bhandarkar and Kshanada J Meshram, Identification and genetic purity testing of cotton F1 hybrid using molecular markers,Indian Journal of Biotechnology,Vol 10(2011) 301-306
[4]. S Liu , R G Cantrell, Jr J C McCarty & J D McStewart, Simple sequence repeat based assessment of genetic diversity in cotton race stock accessions, Crop Sci 40 (2000) 1459-1469.
[5]. O A Gutierrez, S Basu, S Saha, J N Jenkins, D B Shoemaker et al, Genetic distance among selected cotton genotypes and its relationship with F2 performance, Crop Sci 42 (2002) 1841-1847..
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| Paper Type | : | Research Paper |
| Title | : | Isolation and Screening of Lipase Producing Organisms Using De-oiled Seed Cake as a Substrate |
| Country | : | India |
| Authors | : | Asmita R Shringeri || G. R. Naik |
Abstract: Microbial enzymes are often more useful than enzymes derived from plants or animals because of the great variety of catalytic activity. Lipases are the acyl hydrolases and water soluble enzymes that play a role in fat digestion by cleaving long chain triglycerides into polar lipids. Because of an opposite polarity between the enzyme (hydrophilic) and their substrate (lipophilic), lipase reaction occurs at the interface between the aqueous and oil phase. The main purpose of the study is to utilize Seed cake for the production of industrially important enzyme. In this study the soil sample was collected from garage and petrol bunks, screened for lipase producing organisms grown on tributyrin agar media. The isolates showing maximum lipase activity were studied using de-oiled seed cake as substrate for biochemical parameters.
Key words: Lipase, Tributyrin, De-oiled seed cake.
[1] Adebowale, K.O.; Adedire, C.O.; African Journal of Biotechnology, 2006, 5 (10), 901-906.
[2] Altin R, Cetinkaya S; Yucesu HS; J. Energy Convers Manage 2001, 42, 529–38.
[3] Azam, M. M.; Waris, A.; Nahar, N.M., J. Biomass and Bioenergy, 2005, 29, 293-302.
[4] D. Gilham, R. Lehner, Methods, 2005, 36, 139-147.
[5] Ghosh PK, Saxena TK, Gupta R, Yadav RP, Davidson S. Microbial lipases: production and applications. Sci Prog 1996; 79:119 – 57.
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| Paper Type | : | Research Paper |
| Title | : | Media Optimization for Bioethanol Production Using a Native Isolated Strain from Mahua Flowers |
| Country | : | India |
| Authors | : | Rohan Gavankar || Maya Chemburkar |
Abstract: Traditionally mahua flowers are collected by the tribals and used for making liquor is practiced in many states in Northern India. These flowers are rich in sugars and hence harbours native yeast flora capable of carrying out natural fermentation. In the current work previously isolated strain IL2 from Mahua flowers was used for bioethanol production following optimization of physicochemical parameters and nutritional parameters of the media using shake flask fermentation. The bioethanol production for IL2 was 10.65 % (w/v) whereas the reference Saccharomyces cerevisiae MTCC174 was 15.76% (w/v) under optimized conditions. The production of the native isolate can be enhanced by carrying out fermentation in a fermenter and improvising the downstream protocols in future.
Key words: Media optimization, native yeast, Mahua flowers, Bioethanol, fermentation
[1] Swain MR. Kar S. Sahoo AK and Ray RC, Ethanol fermentation of mahua (Madhuca latifolia L.) flowers using free and immobilized yeast Saccharomyces cerevisiae, Microb Res., (2007). 162: 93-98.
[2] Patel JD, Venkataramu K and Rao MS Subba Mahua Madhuca longifolia macb. flowers as raw materials for brandy. Res.Ind., (1982). 27: 251-254.
[3] Gokhale AG. Mahua flowers as a raw material for the acetone fermentation process. J. Indian Insi. Sci., 8: 84-88.
[4] Sutaria BP. and Magar NG. Lactic acid from mowrah flowers. J. Sci.Ind Res. (1958). 17: 136-138.
[5] Sandeep Patil Mandeep Kaur H. K. Sharma Effect of Incorporation of Mahua Extract, Fining Agent and Ageing on the Quality Characteristics of Red Wine, Indian J Microbio., (2012). 52(3) 406-410.
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| Paper Type | : | Research Paper |
| Title | : | Overexpression of ProtochlorophyllideOxidoreductase C (Por C) in Oryza Sativa Var. IR64 |
| Country | : | India |
| Authors | : | Ritu Kumari Singh |
Abstract: India accounts for a total 20% of world rice production. However a significant amount of rice crop is damaged by high incoming irradiance. Thus photo-protective responses are induced in the plant even under otherwise ideal conditions. Further the stress response can greatly escalate if other abiotic factors such as water and nutrients are also limiting. Thus Quantum yield of photosynthesis is greatly reduced under prolonged exposure (hours or days) to high irradiance. It causes damage to the primary photosynthetic reactions and subsequently a reduction in the photosynthetic rate of the crop. It has been observed in rice plants in the dry season even under well-watered, optimal conditions...........
Key words: Chlorophyll molecules, irradiation, photo-protective, transgenic protochlorophyllide, quantum yield
[1]. Affek HP and YakirD(2002) Protection by isoprene against singlet oxygen in leaves. Plant Physiol 129: 269—277
[2]. Armstrong GA, Runge S, Frick G, Sperling U and Apel K (1995) Identification NADPH:protochlorophyllideoxidoreductase A and B: a branched pathway for light-dependent chlorophybiosynthesis in Arabidopsis thaliana. Plant Physiol 108: 1505-151
[3]. Aronsson H, Sundqvist C, Timko MP and Dahlin C (2001a) The importance of the C-terminal region and Cys residues for the membrane association of the NADPH: protochlorophyllideoxidoreductase in pea. FEBS Lett 502: 11--15
[4]. Begley TP and Young H. (1989) Protochlorophyllidereductase. 1. Determination of the regiochemistry and the stereochemistry of the reduction of protochlorophyllide to chlorophyllide. J Am ChemSoc 111: 3095--3096
[5]. Benli M, Schulz R and ApelK (1991) Effect of light on the NADPH-Pchlideoxidoreductase (POR) of A thaliana. Plant MolBiol 16: 615--625
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| Paper Type | : | Research Paper |
| Title | : | Comparative study of Protein enrichment of Lignocellulose Wastes using Baker's Yeast (Saccharomyces cerevisiae) for Animal Feeds |
| Country | : | Nigeria. |
| Authors | : | Kutshik J.R. || Usman, A.M || Ali-Dunkrah U |
Abstract: Obtaining an economical product for food and feed for animals could solve worldwide food protein deficiency. And these can be possible through the bioconversion of fruit wastes into certain valuable products like single cell protein (SCP). However, using wastes as substrate for the production of high nutritious product may also alleviate environmental pollutant up to some extent. In this study, we attempt to use two different wastes as carbon source to see which support Saccharomyces cerevisiaefor the production of SCP more. The sugarcane bagasse and groundnut shells were used as sole carbon source for preparation of fermentation media on which Saccharomyces cerevisiae was grown............
Key words: Single Cell Protein, Sugarcane bagasse, Groundnut shells, Saccharomyces cerevisiae
[1]. AOAC. (2006). The Association of Official Analytial Chemists. The Official Methods of Analysis of AOAC International, 18th ed. Arlington, U.S.A.
[2]. Anupama, D and Ravindra P., (2000). Value added Food: single cell protein. Biotechnology advances, 18, 459- 479.
[3]. DeMartini, J.D., Pattathil, S., Miller, J.S., Li, H., Hahn, M.G. and Wyman, C.E.(2013). Investigating plant cell wall components that affect biomass recalcitrance in poplar and switchgrass. Energy & Environmental Science, 6(3), p.898. Available at: http://xlink.rsc.org/?DOI=c3ee23801f.
[4]. Dhanasekaran, D., Lawanya, S.,Saha, S., Thajuddin, N. and Panneerselvam, A. (2011).Production of Single Cell Protein from Pineapple waste using Yeast.Innovative Romanian Food Biotechnology, Vol. 8. Pp 26 - 32.
[5]. Gad, A. S., Hasan E. A. and Abd E.l Aziz A. (2010).Utilization of Oputina ficus indica waste for production of Phanerochaete chrysosporium bioprotein.J. of American Sci., 6(8).
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| Paper Type | : | Research Paper |
| Title | : | Agrobacterium-Mediated Transformation of PsCBL and PsCIPK Gene in Indica Rice (Oryza sativa L.) |
| Country | : | Bangladesh. |
| Authors | : | Asif Mahmud || Md. Akkas Ali || Shamsul Haque Prodhan |
Abstract: CBL and CIPK genes are play a major role during abiotic stresses through Ca2+ mediated signaling pathway. This study was aimed to investigate efficient Agrobacterium mediated transformation of the two indica rice cultivars viz., BRRI dhan 47 and BRRI dhan 53, independently with PsCBL and PsCIPK gene of pea (Pisum sativum) to enhance abiotic stress tolerance of the rice cultivars. With different hormonal combination tested, the maximum callus induction 85% and 71% was observed on MS medium supplemented with 2 mg/l 2,4-D, 1 mg/l BA and 0.5 mg/l NAA from the mature seeds of BRRI dhan 47 and BRRI dhan 53 respectively......
Key words: 2,4-D, BRRI dhan, callus, CBL, CIPK
[1] Hongbo, S., Zongsuo, L., Mingan, S. Changes of anti-oxidative enzymes and MDA content under soil water deficits among 10 wheats (Triticum aestivum L.) genotypes at maturation stage. Colloids and Surfaces B: Biointerfaces 45, 2005, 7–13.
[2] Tao, H., Brueck, H., Dittert, K., Kreye, C., Lin, S., Sattelmacher, B. Growth and yield formation for rice (Oryza sativa L.) in the water-saving ground cover rice production system (GCRPS). Field Crops Research 95, 2006, 1–12.
[3] Bouman, B.A.M., Peng, S., Castaòeda, A.R., Visperas RM. Yield and water use of irrigated tropical aerobic rice systems. Agriculture and Water Management 74, 2005, 87–105.
[4] Lafitte, H., Bennet, J. Requirement for aerobic rice. Physiological and molecular considerations. Water - Wise Rice Production. IRRI, Los Baños, Philippines. 2003
[5] Witcombe, J.R., Hollington, P.A., Howarth, C.J., Reader, S., Steele, K.A. Breeding for abiotic stresses for sustainable agriculture. Philosophical Transactions of the Royal Society B: Biological Sciences 363, 2008, 703–716.
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| Paper Type | : | Research Paper |
| Title | : | In Vitro Cytotoxicity Studies of Indian Herb Tribulus Terrestris on Human Breast Cancer Cells |
| Country | : | India |
| Authors | : | Apurva Patel || Anjali Soni || Preeti Sharma |
Abstract: Tribulus terrestris (family Zygophyllaceae), commonly known as Gokharu or Punture wine has been used since long in Indian medicine for the treatment of various kind of diseases. The main active compounds of the plant are the steroidal Saponins. The present study aims to analyze the cytotoxic effect of methanolic and Saponin extracts ofTribulus terrestrison human breast cancer line MCF-7. The cytotoxic effect was established by MTT, Sulforhodamine B and LDH cell viability assays............
Key words: Tribulus terrestris, Saponins, Cytotoxicity, Human Breast Cancer cells
[1]. Adaikan, P, G; Gauthaman, k; Prasad, R, N. Proerectile pharmacological effects of Tribulus terrestris extract on the rabbit corpus cavernosum. Annals, Academy of Medicine, Singapore. 229(1), 2000, 22-6.
[2]. Angelova, S., Gospodinova, Z., Krasteva, M., Antov, G., lazanov, V., Markov, T., Bozhanov, S., Georgieva, E., Mitev, V. Antitumor activity of Bulgarian herb Tribulus terrestris L. on human breast cancer cells. Journal of Bioscience and Biotechnology. 2(1), 2013, 25-32.
[3]. Anita, T., Shukla, N.P., Uma devi, P. Comparative study of alcoholic and aqueous extracts of Tribulus terrestris on specific and non-specific immune responses in wistar rats: an in vivo study. International Journal of Pharmacy and Pharmaceutical Science. 5(3), 2013.
[4]. Dinchev D, Janda B, Evstatieva L, Oleszek W, Aslani MR, Kostova I. Distribution of steroidal Saponins in Tribulus terrestris from different geographical regions. Phytochemistry, 69(1), 2008, 176-186.
[5]. Ferlay, J. B.F., Pisani P., Parkin D.M. GLOBOCAN 2000: Cancer Incidence, Mortality and Prevalence Worldwide.Version 1.0. 2001.
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| Paper Type | : | Research Paper |
| Title | : | An Efficient Organogenesis Based Micropropagation of Medicinal and Natural Sweet Leave Plant Stevia Rebaudiana Bert. in Kingdom of Bahrain |
| Country | : | Kingdom of Bahrain |
| Authors | : | Malabika R. Pathak |
Abstract: Stevia rebaudiana Bert. is a small shrub, bearing small leaves which are 30 times more sweeter than sugar. A rapid and efficient plant micro-propagation technique of Stevia rebaudiana based on leaf derived callus induction and shoot differentiation, via individual shoot culture and multiple microshoot induction has developed. Several plant tissue culture media based on modified Murashige and Skoog (MS) medium supplemented with different plant growth regulators (PGRs) were used for callus induction, callus differentiation, microshoot induction and root development. 87% leaf explants showed callus induction in presence of 0.9 μM 2,4-dihydroxyacetic acid (2,4-D),.........
Key words: Growth regulators, Micropropagation, Regeneration, Stevia
[1] Abo El-Nil, M.M (1997). Tissue culture of native plants in the developing countries. Acta Hort. 447:507-512.
[2] Baksha, R M., Jahan, A.A., Khatum R and Munshi, J.L (2005). Micropropagation of Aloe barbadensis Mill. through in vitro culture of shoot tip explants. Plant Tissue Culture and Biotech 15:121-126.
[3] Bespalhok, J.C.F and Hattori, K (1997). Embryogenic callus formation and histological studies from Stevia rebaudiana (Bert.) Bertoni floret explants. Rev Bras Fisiol Veg 9 (3):185-188.
[4] Bhatt, I.D and Dhar, U (2000). Micropropagation of Indian wild strawberry. Plant Cell Tiss Org Cult 60:83-88.
[5] Brandle, J.E., Starratt, A.N and Gijzen, M (1998). Stevia rebaudiana: Its agricultural, biological and chemical properties. Canad J Plant Sci 78:527-536.
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| Paper Type | : | Research Paper |
| Title | : | Identification of pork (susscrofa), horse (Equuscaballus) and donkey (Equusasinus) by PCR-RFLP-DHPLC analysis on the mitochondrial COI gene |
| Country | : | China |
| Authors | : | Liping Song || Jie Jiang || Ting Mao || Na Shi || Wei Li || Jie Hao || Zhikai Hu || Miao Guo |
Abstract: Donkey flesh is a popular food in China. To ensure the quality of donkey products plays a vital role in protecting the interests of consumers. Pork and horse are potential in donkey flesh food because of their easier availability at cheaper prices. In present study, PCR-RFLP-DHPLC technology was established for distinguishing the pork, horseflesh and donkey flesh in donkey flesh food. The assay combined the species-specific primers to selectively amplify a short fragment of mitochondrial cytochrome c oxidase subunit I (COI) gene from a heterogeneous background of genomic DNAs followed by RFLP-DHPLC analysis........
Key words: PCR-RFLP-DHPLC, donkey, COI gene
[1]. Wang, Q., et al., Identification of 12 animal species meat by T-RFLP on the 12S rRNA gene. Meat Sci, 2010. 85(2): p. 265-9.
[2]. Chisholm, J., et al., The detection of horse and donkey using real-time PCR. Meat Sci, 2005. 70(4): p. 727-32.
[3]. von Bargen, C., J. Brockmeyer, and H.U. Humpf, Meat authentication: a new HPLC-MS/MS based method for the fast and sensitive detection of horse and pork in highly processed food. J Agric Food Chem, 2014. 62(39): p. 9428-35.
[4]. Zhao, C.J., et al., Differentiating among horse (Equus caballus), donkey (Equus asinus) and their hybrids with combined analysis of nuclear and mitochondrial gene polymorphism. J Anim Breed Genet, 2005. 122(4): p. 285-8.
[5]. Soares, S., et al., Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Sci, 2010. 85(3): p..
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| Paper Type | : | Research Paper |
| Title | : | Wildlife forensic techniques: DNA Extraction from hard Biological matter ofChital (Axis axis), molecular analysis,use of SEM andEDXin species identification |
| Country | : | India. |
| Authors | : | Amit Kumar Verma || Shahbaz Khan || P.C Joshi || Archana Bahuguna || Kapil Dev |
Abstract: India is globally recognised as Mega bio-diverse nation, encompassing plethora of floral and faunal species within its numerous Eco-zones. This wildlife treasure is currently threatened to extinction by rampant poaching and illegal trade. DNA identification of species unravels potential possibilities in the field of conservation biology. The information, database on genetic diversity of wildlife is necessary to as certain genetically deteriorated populations in order to formulate better conservation prospects. Accurate identification of the threatened species not only provides understanding of their morphological characteristics but also the existing state of their vulnerability and prospects for their conservation........
Key words: Wild life, Forensic techniques, Biodiversity, conservation, Chital, DNA Extraction, PCR, SEM, EDX.
[1]. Ogden R, Dawnay N, McEwing R Wildlife DNA forensics—bridging the gap between conservation genetics and law enforcement.Endang. Spec. Res. (9), 1999, 179–195.
[2]. Sahajpal.V and Goyal. SP ,Identification of a forensic case using Microscopy and forensically informative nucleotide sequencing (FINS), Acase study of small Indian civet (Viverricula indica), Science and Justice, scijus.2009.07-09.
[3]. Steroid B, Fisher RA, Khan IM, Kessling AM, Archard LC,et al.Conserved gene sequences for species identification: PCR analysis of the 3' UTR of the SON gene distinguishes human and other mammalian DNAs. Forensic Sci Int 1995,73:171–8.
[4]. Chaw YFM, Crane LE, Lange P, Shapiro R, Isolation and identification of cross-links from formaldehyde-treated nucleic acids. Biochemistry 19,1980,5525–5531.
[5]. Paabo S, Higuchi RG, Wilson AC,Ancient DNA and the polymerase chain reaction: the emerging field of molecular archaeology. J. Biol. Chem. 264, 1989,9709–9712..
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| Paper Type | : | Research Paper |
| Title | : | Synthesis and Spectroscopic Characterization of Cr (II) –2-hydroxy-1, 2-phenylethanone Complex |
| Country | : | India. |
| Authors | : | Man Mohan Singh Jassal |
Abstract: The complex of benzoin with chromium (II) ion was prepared and was characterized by infrared, electronic spectra and elemental analysis. The spectroscopic analysis showed the presence of metal ion in the complex and showed the formation of complex in a 1:1 [M: L] ratio with formula as [Cr (L) (OH) (H2O) 3]. 3H2O, where L represents the Benzoin. The IR spectral analysis showed that the complex formation takes place via oxygen atoms of the carbonyl and hydroxyl groups of 2-hydroxy-1, 2-phenylethanone moiety. The Electronic spectral data showed that the complex formation showed a typical geometrical structure of Cr (II) complex, and the magnetic moment analysis showed the paramagnetic behaviour of the complex, however the molar conductance values so obtained reveals the non-electrolyte nature of the complex.
Key words: 2-hydroxy-1, 2-phenylethanone, Chromium, Spectroscopy.
[1]. A.A. Maihub, M.M. El-ajaily, M.A. Abouzweda, H.F. El-amari and E.S. Ahmed, "Synthesis Of Some Mixed Ligand Complexes Derived From Catechol And 2- Aminopyridine And Their Biological Activity" journal of basic and applied Sciences, 15(1), 41-49(2005).
[2]. Offing O.E., Martelli S. Antibacterial Activity of Metal Complexes of Benzil and Benzoin Thiosemicarbazones, IL Farmaco., 49 (1994) 513-518.
[3]. B.K. Mohanty, R.M. Mohapatra, N. Millick and B.K. Mohapatra; synthesis and the characterization of the iron-benzoin complexes, Indian Journal of Chemistry, 26A, 1069(1987).
[4]. Londa Borerpis, Linda Thalken, Christopher Ceccarelli, Synthesis and characterization of a hydroxyl-bridged iron(III) dimer of N,N'-ethylenebis(salicylamine). Inorg. Chem. 22(12), 1983, 1719–1724.
[5]. Vogels, "Textbook of practical Organic Chemistry", 5th Ed., Longmans. London (1989)..