Abstract: Effects of heavy metal pollution on the enzymatic activities of soils were investigated in the Ishiagu mining area of Ebonyi State, Nigeria. Soil dehydrogenase, polyphenol oxidase, hydrogen peroxidase, alkaline and acid phosphatases and urease were measured to evaluate the effects of heavy metals mining operations on soil biochemical characteristics. Results showed that the soil pH ranged from 5.04 to 6.56 while the heavy metals were of the ranges: Pb (13,754-29,491 mg/kg), Zn (1,151-2,778 mg/kg), Cd (17.65-27.71 mg/kg), Cu (9.59-39.91 mg/kg) and total heavy metals (014,932.24-32,336.62 mg/kg). Soil heavy metals concentrations significantly decreased with the increase of distance from the mining pit while soil pH increased with the increase of the distance from the mining pit. Analysis of the soil enzyme activities indicated a significant positive correlation at P≤ 0.05 between soil enzyme activities and soil pH. On the contrary, the activities of dehydrogenase, polyphenol oxidase, hydrogen peroxidase, alkaline and acid phosphatases and urease showed significant negative correlation at P≤ 0.05 with the heavy metal contents except for Zn against dehydrogenase activity and Cd against hydrogen preoxidase and urase activities that were though negative but statistically not significantly correlated at P≤ 0.05. This showed that the activities of the enzymes analysed could be used as sensitive indicators of heavy metals contamination. The results in general indicated that the mining operations at Ishiagu, Ebonyi State affect the soil quality due to heavy metal contamination
Key words: Enzyme activities, heavy metals, Ishiagu, soil, pollution.
[1] Ebong, G.A., Etuk, H.S., and Johnson, A.S. (2007). Heavy metal accumulation by Talinum Triangulare grown on waste dumpsite in Uyo metropolis, Akwa-Ibom State, Nigeria. Journal of Applied Sciences 7(10): 1404-1409.
[2] Ololade, I.A., Ashoghon, A.O., and Adeyemi, O. (2007). Plants level of Chromium and Nickel at a refuse site. Any positive impact? J. Appl. Sci. 7(13):1768-1773.
[3] Lee, I., Kim, O.K., Chang, Y., Bae, B., Kim, H.H., and Baek, K.H. (2002). Heavy metal concentrations and enzymatic activities in soil from contaminated Korean shooting range. J. Biosci. Bioeng., 94 (5): 406-411.
[4] Ma L.Q., Tam. F., and Harris, W.G. (1997). Concentration and Distribution of eleven metals inFlorida soil. J. Environ. Qual., 26:769-775.
[5] Dulka, S., Piotrowska, M., Chlopecka, A., and Witek, T. (1995). Trace element contamination of soils and crop plants by mining smelting industry in southwest Poland. J. Geochem. Explor. 52:232-250.
[6] Dulka, S. (1992). Factor analysis of total element concentrations in Surface soils of Poland. Sci.Total Environ, 121:39-52.
[7] Stuczynski, T.I., McCarty, G.W., and Siebielec, G. (2003). Response of Soil Microbiological activities to cadmium, lead and zinc salt amendments. J. Environ. Qual., 32:1346-1355.
[8] Doelmann, P., and Haanstra, L. (1984). Short-term and long-term effects of Cd, Cr, Cu, Ni, Pb and Zn on microbial respiration in relation to abiotic soil factors. Plant and soil, 79: 317- 321.
[9] Chaney, W.R., Kelly, J.M., and Strickland, R.C. (1978). Influence of Cd And Zn on carbon dioxide evolution from litter and soil from a black forest. J. Environ. Qual., 7: 115-119.
[10] Koper, J., Dabkowska-Naskret, H., and Piotrowska, A. (2005). Influence of heavy metals on enzymatic activity in lessive soils of Kujawy and Pomoroze region (Poland). Geophysical Research Abstracts, 7:10565-10569.