Abstract: Listeria monocytogenes is a pathogenic bacteria of great concern in the food industry due to its wide distribution in the environment, its ability to grow at low temperatures and relatively high salt levels, and the potential severity of the infection with high mortality, mainly in pregnant women, the elderly and immunocompromised patients. Food-borne listeriosis remains a public health threat. One hundred forty two minced raw meat samples were collected from various points (processing plants, hotels, restaurants, retail outlets, caterers and wholesalers) and were analyzed for the detection of L. monocytogenes by conventional culture and Real-time polymerase chain reaction (Real-time PCR). Our results showed frequent contamination of raw minced meats (21%). Our statistical analysis showed a relatively correlation (R2 = 0.637, p <0.01) between the two methods, while the sensitivity, specificity, and positive and negative PCR method predictive values were respectively 30, 92, 37 and 97%. Continuous environmental monitoring schemes for L. monocytogenes are of major importance to identify potential contamination sources and as an early warning system for meat business operators. Hence, the detection of L. monocytogenes by Real-time PCR method allows good control of the health risk.
Keywords: Control, Health risk, Listeria monocytogenes, Minced raw meat, Real-time PCR
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