Abstract: Level of homogeneity and variation of S. afra populations collected from coastal waters of Lagos state were studied using random amplified polymorphic DNA (RAPD)-PCR amplification. Fish specimens obtained from Oshoroko, Orimedu, Lagos Lagoon, Ibese and Yovoyan stations were used for the experiment. DNA was isolated using salting out method from caudal fin of the fish and RAPD analysis was carried out with five 10 bp- oligo- nucleotide random primers (RAPD). Four out of the five primers showed stable amplification and polymorphism with ampicons.............
KeyWords: Random amplified polymorphic DNA (RAPD), polymorphism, primers, Sphyraena afra, genetic similarity.
[1]. Ahmad J.N., Azizah, M.N., Adikwu, I.A., Istifanus, W. A.,Abalis,G.E and Muchlisin,Z.A (2012). Diversity and distribution of fishes of Gaji River, Bauchi state Nigeria. Advances in Environmental sciences-International journal of the bioflux society vol 4, issue 2. Pp.50 -54.
[2]. Bardakci, F. and Skibinski, D.O.F (1994). Application of the RAPD technique in tilapia fish: species and subspecies identification Heredity 73:117 – 123.
[3]. Barman, H.K, Barat, A., Ydav, B.M., Banerjee, S., Meher, P.K., Reddy, P.V. and Jana, R.K. (2003). Genetic variation between four species of Indian carp as revealed by random amplified polymorphic DNA assay. Aquaculture 217: 115 – 123.
[4]. Bártfai, R., Egedi, S., Yue, G.H., Kovács, B., Urbányi, B., Tamás, G., Horváth, L. and Orbán, L. (2003). Genetic analysis of two common carp broodstocks by RAPD
[5]. Beacham, T.D., Hay, D.E. and Le, K.D. (2005). Population structure and stock identification of Eulachon (Thaleichthys pacificus), an anadromous smelt in the Pacific Northwest. Marine Biotechnology, 7: 363 – 72.