Abstract: In this experimentcollagen was extracted from the squid scales by water extraction. The content of collagen in the extract was determined by spectrophotometry. The effects of extraction temperature, ratio of material to liquid, pH value and extraction time on the extraction rate of collagen in carp scales were studied. From the single factor test, The better parameters for extracting collagen by water extraction were extraction temperature of 20~30°C, ratio of material to liquid of 1:20~1:30 (g/mL), pH of 5~6 and extraction time of 5h. Based on the above, three factors including extractiontemperature, solid-liquid ratio and pH value which had great influence on the extraction rate of collagen were selected to carry out orthogonal test. The optimum process parameters for water extractionof collagenwere as follows: extraction temperature of 20 °C, pH value of 6 and the ratio of material to liquid of 1:20 (g/mL)..
Keywords: Carp scale of, Collagen, Water extraction
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