Abstract: The biochemical and histopathological alterations in the blood and liver of the teleost fish, Anabas testudineus subjected to two sub-lethal concentrations of copper and the capacity of vitamin C to counteract the toxicity induced perturbations were investigated. The 96 hour LC 50 value of copper for A. testudineus was determined by Probit method and was found to be 1.74 mg/L. The blood and liver samples were collected from copper exposed and vitamin supplemented media on the 7th,14th,21stand 28thday of exposure. Copper exposure significantly increased (p˃0.0001) the activity of biomarkers of hepatotoxicity such as GOT (Glutamate oxalate transaminase) and GPT (Glutamate pyruvate transaminase) in serum and liver compared to vitamin supplemented fishes.The decrease in serum and liver protein of copper exposed fishes justifies increased energy demand due to copper induced oxidative stress. The liver of copper treated fish showed histopathological alterations such as degeneration of hepatocytes,cell necrosis, inflammation with sinusoid dilation,increased incidence of kupffer cells and vacuole formation. Vitamin administration was effective in improving the physiological and histological integrity in copper intoxicated fishes.The present study shows that copper is harmful to Anabas testudineus even at sub-lethal concentrations and it's enrichment in water and sediment is a dangerous threat to aquatic life.
Keywords: Copper,GOT,GPT, histopathology, Vitamin C
[1]. Abdel-Meguid.,N,A.M.Kheiralleh.,Abu-Shaban,K.,Adham,A. and Abdel-Moneim, Histochemical and biochemical changes in liver of Tilapia Zilli: as a consequence of water pollution. Journal of Biological Science, 2, 2002, 224 – 229.
[2]. S.S.Vutukuru, Acute effects of hexavalent chromium on survival, oxygen consumption, haematological parameters and some biochemical profiles of the Indian major carp, Labeo rohita. International Journal of Environmental Research and Public Health. 2(3), 2005, 456 – 462.
[3]. Randall,D.J. and S.F. Perry, Catecholamine In. Hoar, W.S., Randall, D.J. and T.P.Farrell, (Ed.), Fish physiology, Vol. XII, (Academic press, New York, 1992)
[4]. Barton, B.A. and G.K.Iwama, Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual Reviews of Fish Diseases (1), 1991, 3-26.
[5]. Beggs, K. and N.W..Pankhurst, Endocrine and metabolic responses to stress in a laboratory population of the tropical damselfish, Acanthochromis polyacanthus. Journal of Fish Biology. (64),2004, 133-145.