Abstract: A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of flubendiamide and its metabolite (flubendiamide- des- iodo) residues. The evaluated parameters include the extracts by alumina packed column using hexane: ethyl acetate solvent mixture (9:1) and acetonitrile solvent. The method was validated using leaf samples spiked with flubendiamide and its metabolite (flubendiamide- des- iodo) at different fortification levels (0.03 and 0.3 μg/g). Average recoveries (using each concentration six replicates) ranged 85-96%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.03-10.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01μg/g and 0.03μg/g respectively. Finally the leaf residue samples were re analyzed by HPLC. Key words: HPLC, Alumina, Flubendiamide and its metabolite and jatropha plant leaves.
[1]. Keith Openshaw, Areview of jatropha curcas: an oil plant of unfulfilled promise, Biomass and Bio energy, 19(1), 2000, 1-15.
[2]. D.M Reddy Prasad, Amirah Izam and Md. Maksudur Rahman Khan, Jatropha curcas: Plant of medical benfits, Journal of medicinal
plants research, 6(14), 2012, 2691-2699.
[3]. Rubaljot Kooner, S.K.Sahoo, Balwinder Singh and R.S. Battu, Dissipation kinitics of flubendiamide and thiacloprid on tomato and
soil, Quality assurance and safety of crops & foods, 2(1), 2010, 36-40.
[4]. S. Mohapatra, A.K. Ahuja, G.K. Jagadish, N. Rashmi, D. Sharma, Development of an analytical method for analysis of
flubeniamide, des-iodo flubendiamide and study their residue persistence in tomato and soil, J Environ Sci Health B, 46(3), 2011,
264-271.
[5]. Paramasivam, M, Benerjee, Hemantha, Simultaneous determination of flubendiamide its metabolite desiodo flubendiamide residues
in cabbage, Tomato and pigeon pea by HPLC, Bulletin of environmental contamination &Toxicology, 87 (4), 2011, 452.