iosr-JPBS   Volume-12 ~ Issue-6 ~ Nov-Dec 2017

Abstract: Moringa oleifera, is commonly known as 'drumstick tree' or 'horseradish tree'. Every part of the plant has high nutritive value. In this study the leaf extracts of Moringa oleifera was prepared using 7 different solvents and phytochemically analysed in which methanol leaf extract showed presence of many compounds. Quantification of leaf extract showed presence of high amount tannins. Palmitic acid was isolated from the extract and antimicrobial activity of palmitic acid was studied at different concentration for both bacteria and fungi. The results revealed that though both Gram negative and positive bacterial species were inhibited the gram negative were resistant particularly P. aeruginosa and high zone of inhibition was observed with K. pneumonia. The plant has many pharmacological properties and need the scientific values from the researcher for commercialization

Keywords: Enzyme immobilization; Thermomyceslanuginosus lipase (TLL), carbon nanotube, magnetic nanoparticles, Applications.

Abstract: The Study entitled "A CROSS SECTIONAL STUDY ON BELIEFS, ATTITUDES, MISCONCEPTIONS AND KNOWLEDGE OF PATIENTS & COMMUNITY TOWARDS LEPROSY IN AND AROUND RURAL AREAS OF GUNTUR DISTRICT" was designed to assess the knowledge of Leprosy in and around rural areas of Guntur district, Andhra Pradesh, India, and also to evaluate the impact of an educational intervention carried out in this study. A suitable self-administered (KAP) Knowledge, attitude, practice survey questionnaire was designed and validated by this study and the study was conducted among the patients and community in that area. This cross sectional knowledge attitude practice (KAP) questionnaire study, of 6 month duration included a total of 207 participants out of 403............

Keywords: Hansen's disease, Patients & community people, KAP questionnaire

Abstract: Biosurfactants are surface active compounds that reduce the interfacial tension between two liquids or between a liquid and solid, these compounds produce extracellularly or as part of the cell membrane in bacteria, yeast and fungi. In this study the Pseudomonas putida PS6 isolated from soil contaminated with hydrocarbons and it showed the highest capability to produce the biosurfactant when grown in BH media with 1% of diesel oil as carbon source. The biosurfactant was extracted from cell-free supernatant of Pseudomonas putida PS6 culture by chloroform: methanol (2:1) and then identified based on the methods of TLC, chemical compositions, blue agar plate and FTIR analysis. The results from all methods indicate that the biosurfactant can designated as glycolipid..............

Keywords: Pseudomonas putida, biosurfactant, glycolipid

Abstract: The current study aims to investigate the relationship between lipid peroxidation, represented by seminal malondialdehyde (MDA) level, and integrity of sperm plasma membrane, represented by the hypo-osmotic swelling (HOS) test score, in infertile men. One hundred and twenty Iraqi men (20 fertile and 100 infertile) shared in this study during their attendance to the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies/Al- Nahrain University. The mean age of the fertile men was (30.46±0.78) years while the mean age of infertile men was (32.58±0.64) years with the duration of infertility (5.36±0.33) years.............

Keywords: Hypo-osmotic swelling test, Infertile men, Lipid peroxidation, Malondialdehyde

Abstract:A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Cilnidipine and Chlorthalidone, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6 x 150mm, 5μm) column using a mixture of ACN, Methanol and Phosphate buffer pH4.6 (10:25:65 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 234nm. The retention time of the Cilnidipine and Chlorthalidone was 2.088, 6.068 ±0.02min respectively. The method produce linear responses in the concentration range of 25-125ppm of Cilnidipine and 10-50ppm of Chlorthalidone. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords: Cilnidipine, Chlorthalidone, RP-HPLC, validation.

Abstract: Cancer is one of the most common devastating disease affecting millions of people per year. Cancer has been estimated as the second leading cause of death in humans. So there has been an intense search on various biological sources to develop a novel anti-cancer drug to combat this disease. In this review we describe and discuss several approaches to selecting higher plants as candidates for drug development with the greatest possibility of success. We emphasize the role of information derived from various systems of traditional medicine (ethnomedicine) and its utility for drug discovery purposes. Plants have proved to be an important natural source of anti-cancer therapy for several years.............

Keywords: Anti-cancer, Herbal medicine, Clinical trials, Cancer treatment

Abstract: Background:Ureteric colic is commonly diagnosed and treated in the emergency department [1] .It is a large worldwide [2]. It is caused by acute partial or complete ureteric obstruction due to a calculus in the vast majority of cases [3]. Many factors have been suggested to contribute to the occurrence of renal & ureteric stones, including diet, water hardness, geographical location, race, occupation, sex, and climatic conditions [7–9] . Aim of the study:to evaluate the demographic , clinical characteristics and management of patients presented with ureteric colic per one year . Subjects & Methods:This study was carried out in the surgical emergency department of Baghdad Teaching Hospital of Medical City through one year ( from 01/12/2011 to 30/11/2012 ). Any patient older than 13 years of age presented to the surgical emergency department.............

Keywords: Urolithiasis, ureteric colic, emergency department

Abstract: The frond of Albizia chinensis(osbeck)merr extract was obtained from the powder using 80% methanol. Preliminary phytochemical works were carried out for detection of secondary metabolite. Estimation of alkaloids, flavonoids, protein, saponins, was carried out using appropriate test. A thin layer chromatographic technique was used for compound separation and identification from the extract. The total ash, acid insoluble ash, sulphate ash, water soluble ash values of entire plants of control and commercial raw drugs have been revealed. The control of total ash values of entire plant Albizia chinensis is 25mg, 23.19mg, 63mg, 0.52mg, and 65.5mg. The fluorescence character of entire plant Albizia chinensis was undergone in day light and UV 254nm. The extract was subjected to GC-MS analysis and 10 compounds shown use activities. 10 therapeutically active compounds present in the extract. This compound identification could provide valuable information for the preparation of cataract medicine from frond bark of Albizia chinensis (osbeck) merr.

Abstract: Diversity of seeds based on their morphology across vegetation and seasons in Shivan Malai of Salem district, Tamilnadu India was studied in the present research. Fruit type and colour, seed number per fruit, length and breadth of seed, seed weight, seed sculpturing pattern, seed shape, seed colour, hilum, modification of seed / fruits were studied. The study resulted in 11 different types of fruits dominated with capsule and 22 different shapes of seeds dominated with ovate shape, 4 different coloured seeds (yellow, reddish black, pale yellow and greenish brown) dominated with black and brown coloured seeds, 12 different types of seed sculpturing pattern (chagrenate, Scabrate, Reticulate, Echinate, Regulate, Cicatricose, Corrugate, Baculate, Striate, Piliferous, Granular.............

Keywords: Seed, fruit, Colour, shape, hilum, etc.