Abstract: One yeast and three fungal species were isolated from oil mill waste and was identified as Millerozyma farinosa, Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus. The yeast species was isolated on Mannitol yeast agar and the fungal species on Sabouraud dextrose agar. The biosurfactant was produced through submerged fermentation in minimal salt media broth supplemented with 2%dextrose and the biosurfactant screened using various methods. The biosurfactant was characterized. Millerozyma farinosa yielded maximum positive results and the biosurfactant produced by it was purified using dialysis membrane and then analysed using Thin layer chromatography and Fourier transform infrared spectroscopy. The optimum growth conditions were standardized using the factors of pH, temperature, carbon and nitrogen source. It was measured using the drop weight quantitative....
Keywords: Glycerophospholipid, Yeast, TLC,FTIR
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