Abstract: Calcium oxide (CaO) nanoparticle (NP) was synthesized by solution combustion method using urea as fuel. The CaO nanoparticle was characterized by X-ray diffraction (XRD), Scanning Electron Microscope (SEM) analysis and UV-absorption spectral studies. The average crystallite size was found to be ≈36nm and the band gap energy of CaO nanoparticle was 4.9 eV. The toxicity tests were performed on fresh water fish Cyprinus carpio for synthesized CaO NP at different concentrations ranging from 200 to 280 mg/L for 4 days. Bioassays were repeated three times and the data obtained were statistically evaluated using Finney's Probit Analysis. Toxicity test performed revealed that LC50 value for Cyprinus carpio was 234.9mg/L with 95% of confidence limits for 226.819- 242.624mg/L.
Keywords: Calcium oxide, Cyprinus carpio, LC50, SEM, Toxicity, XRD.
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