Paper Type |
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Research Paper |
Title |
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Bacterial Diversity towards Industrially Important Enzyme Producers from Velliangiri Hills, Western Ghats |
Country |
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India |
Authors |
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Logeswaran. R, Prabagaran. S. R. P , Ramesh. D |
|
: |
10.9790/2402-08554563 |
Abstract: The Velliangiri hill of Western Ghats in Tamilnadu was chosen for a comprehensive study on bacterial diversity because there was no previous report on bacterial biodiversity from this region. Soil samples were collected from 7 sub hills, Velliangiri hills. Culture dependent studies were performed to explore bacterial species from collected samples. Overall 101 bacterial isolates were isolated based on their characteristical and microscopical observations. Statistical analysis (Shannon & Simpson) was recorded. From the results of statistical analysis the bacterial colonies were dominant and more diverse in the Aandisunai hill and less diverse in Paambaatti hill. The Simpson's index is always reciprocal to Shannon index. The bacterial isolates which were isolated from Velliangiri hills had the ability to produce different enzymes like amylase, cellulase, pectinase, proteinase, and lipase. Among the isolates, the 10 enzyme producing bacterial cultures were screened based on their ability to produce more than two enzymes. Based on the protein profiling techniques (using MALDI analysis), the isolates were identified. They are Bacillus mycoides, Rhodococcus equi, Sphingomonas koreensis, Bacillus subtilis, Clostridium clostridioforme, Bacillus mojavensis, Pseudomonas alcaligenes, and Paenibacillus glucanolyticus. Among the bacterial isolates, Pseudomonas alcaligenes alone produces all the five enzymes. Hence, the diverse environment of Velliangiri hills provides sufficient diversity to explore the place for various microorganisms for bioprospecting.
Keywords: Velliangiri hills, bacterial diversity, statistical analysis, enzyme producer, MALDI
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